![]() The thickness of the band corresponds to the amount of protein present thus doing a standard can indicate the amount of protein present. As the antibodies only bind to the protein of interest, only one band should be visible. ![]() The bound antibodies are then detected by developing the film. The unbound antibody is washed off leaving only the bound antibody to the protein of interest. ![]() The membrane is then incubated with labels antibodies specific to the protein of interest. These results are then transferred to a membrane producing a band for each protein. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. Western blot is often used in research to separate and identify proteins.
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